Protocatechuate 3,4-Dioxygenase
نویسندگان
چکیده
منابع مشابه
Reaction mechanism of protocatechuate 3,4-dioxygenase.
We have observed that high SOD-like function (decomposition of superoxide anion) was observed for several iron(III) compounds with tripodal ligands and several oxovanadium(IV) compounds, and also that these compounds exhibit high catalytic activity for oxidative cleavage of 3,5-di-tert-butylcatechol in non-donating solvents such as dichloromethane or nitromethane. These are suggesting that the ...
متن کاملOxygenated form of protocatechuate 3,4-dioxygenase, a non-heme iron-containing dioxygenase, as reaction intermediate.
A short-lived new spectral species of protocatechuate 3,4-dioxygenase, a trivalent non-heme iron-containing enzyme, was observed in the early stage of the reaction. This new spectral species was characterized by a broad absorption band with a maximum between 500 and 520 rnp, distinct from those of the enzyme or the enzyme-protocatechuic acid complex. It could be demonstrated only in the presenc...
متن کاملSpontaneous mutations in pcaH and -G, structural genes for protocatechuate 3,4-dioxygenase in Acinetobacter calcoaceticus.
Bacteria containing spontaneous null mutations in pcaH and -G, structural genes for protocatechuate 3,4-dioxygenase, were selected by exposure of an Acinetobacter calcoaceticus strain to physiological conditions in which expression of the genes prevents growth. The parental bacterial strain exhibits high competence for natural transformation, and this procedure was used to characterize 94 indep...
متن کاملRoles of the equatorial tyrosyl iron ligand of protocatechuate 3,4-dioxygenase in catalysis.
The active site Fe(III) of protocatechuate 3,4-dioxygenase (3,4-PCD) from Pseudomonas putida is ligated axially by Tyr447 and His462 and equatorially by Tyr408, His460, and OH(-). Tyr447 and OH(-) are displaced as protocatechuate (3,4-dihydroxybenzoate, PCA) chelates the iron and appear to serve as in situ bases to promote this process. The role(s) of Tyr408 is (are) explored here using mutant ...
متن کاملInfluence of metal ions on bioremediation activity of protocatechuate 3,4-dioxygenase from Stenotrophomonas maltophilia KB2
The aim of this paper was to describe the effect of various metal ions on the activity of protocatechuate 3,4-dioxygenase from Stenotrophomonas maltophilia KB2. We also compared activity of different dioxygenases isolated from this strain, in the presence of metal ions, after induction by various aromatic compounds. S. maltophilia KB2 degraded 13 mM 3,4-dihydroxybenzoate, 10 mM benzoic acid and...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)45088-6